Thebaine Synthase: A New Enzyme in the Morphine Pathway in Papaver Somniferum
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چکیده
منابع مشابه
Acetyl coenzyme A:salutaridinol-7-O-acetyltransferase from papaver somniferum plant cell cultures. The enzyme catalyzing the formation of thebaine in morphine biosynthesis.
Acetyl coenzyme A:salutaridinol-7-O-acetyltransferase, a highly substrate-specific enzyme, has been purified nearly 3,000-fold to homogeneity from Papaver somniferum plant cell suspension cultures. Purification was achieved by fractionated ammonium sulfate precipitation, dye-ligand affinity chromatography on matrex red A, gel filtration, ion exchange chromatography on Mono Q and a second dye-li...
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Opium poppy (Papaver somniferum L.) produces a large variety of isoquinoline alkaloids. The aim of this investigation is to understand the regulation of biosynthesis and the ecological function of the alkaloids in the plant. Agrobacterium-mediated transformations of opium poppy were used to introduce the berberine bridge enzyme cDNA bbe 1 in the antisense orientation into seedling explants. Aft...
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Alkaloids in the terminal capsule of M-89 variety of P. so m n if e rum rise and plateau as the plant matures in a controlled environment. Lancing immature capsules does not increase yield of codeine plus morphine in the above-ground plant, but lancing may translocate alkaloids upward to the capsule. Poppies produced a high proportion of codeine relative to m?rphine. This effect is probably due...
متن کاملRedesign of a dioxygenase in morphine biosynthesis.
Opium poppy (Papaver somniferum) produces medicinally important benzylisoquinoline alkaloids, including the analgesics codeine and morphine, in the morphinan pathway. We aligned three dioxygenases that were recently discovered in P. somniferum and subsequently identified the nonconserved regions. Two of these enzymes, codeine O-demethylase (PsCODM) and thebaine O-demethylase (PsT6ODM), are know...
متن کاملRecent Advances in the Chemistry of Oripavine and Its Derivatives
Oripavine is the major alkaloid of Papaver orientale. It is an important intermediate in the biosynthesis of morphine alkaloids. Recently, new Papaver somniferum strains have been developed which accumulate thebaine and oripavine, but not morphine and codeine. Therefore, the chemistry of oripavine has been studied intensively to synthesize opioid pharmaceuticals such as oxymorphone, naloxone an...
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ژورنال
عنوان ژورنال: Natural Product Communications
سال: 2007
ISSN: 1934-578X,1555-9475
DOI: 10.1177/1934578x0700200305